› 3jyz

X-ray diffraction
1.55Å resolution

Crystal structure of Pseudomonas aeruginosa (strain: Pa110594) typeIV pilin in space group P41212

Released:
DOI: 10.2210/pdb3jyz/pdb
PDB entry 3jyz coloured by chain, front view.
PDB entry 3jyz coloured by chain, side view.
PDB entry 3jyz coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural characterization of novel Pseudomonas aeruginosa type IV pilins.
Nguyen Y, Jackson SG, Aidoo F, Junop M, Burrows LL
J Mol Biol 395 491-503 (2010)
PMID: 19895819

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pilin Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 15.44 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KQ36 (Residues: 34-178; Coverage: 82%)
Gene name: pilA
Sequence domains: Pilin (bacterial filament)
Structure domains: Glycoprotein, Type 4 Pilin

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P41212
Unit cell:
a: 63.059Å b: 63.059Å c: 67.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.209
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pseudomonas aeruginosa twitching motility: type IV pili in action.
Burrows LL. (2012)
5 more

1 mention without citation

Expression, purification, crystallization and preliminary crystallographic analysis of PilA from the nontypeable Haemophilus influenzae type IV pilus.
Kolappan et al. (2012)