› 3k32

X-ray diffraction
2.5Å resolution

The crystal structure of predicted subunit of tRNA methyltransferase from Methanocaldococcus jannaschii DSM

Released:
DOI: 10.2210/pdb3k32/pdb
PDB entry 3k32 coloured by chain, front view.
PDB entry 3k32 coloured by chain, side view.
PDB entry 3k32 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Wu R, Zhang R, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein MJ0690 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 203 amino acids
Theoretical weight: 23.45 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q58102 (Residues: 1-200; Coverage: 100%)
Gene name: MJ0690
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 71.669Å b: 121.951Å c: 81.224Å
α: 90° β: 116.7° γ: 90°
R-values:
R R work R free
0.181 0.177 0.263
Expression system: Escherichia coli BL21

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