› 3kfm

X-ray diffraction
2.2Å resolution

Crystal Structure of the GluA4 Ligand-Binding domain L651V mutant in complex with kainate

Released:
DOI: 10.2210/pdb3kfm/pdb
PDB entry 3kfm coloured by chain, front view.
PDB entry 3kfm coloured by chain, side view.
PDB entry 3kfm coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Enhanced efficacy without further cleft closure: reevaluating twist as a source of agonist efficacy in AMPA receptors.
Birdsey-Benson A, Gill A, Henderson LP, Madden DR
J Neurosci 30 1463-70 (2010)
PMID: 20107073

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148618 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 4 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19493 (Residues: 416-528, 654-795; Coverage: 29%)
  • Best match: P19493-2 (Residues: 416-528, 654-795)
Gene names: Glur4, Gria4
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand KAI 1 x KAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P2
Unit cell:
a: 45.69Å b: 48.445Å c: 62.834Å
α: 90° β: 106.62° γ: 90°
R-values:
R R work R free
0.19 0.19 0.274
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
4 more

1 mention without citation

Enhanced efficacy without further cleft closure: reevaluating twist as a source of agonist efficacy in AMPA receptors.
Birdsey-Benson et al. (2010)