› 3kfq

X-ray diffraction
1.99Å resolution

Unreduced cathepsin V in complex with stefin A

Released:
DOI: 10.2210/pdb3kfq/pdb
Model geometry
Fit model/data
PDB entry 3kfq coloured by chain, front view.
PDB entry 3kfq coloured by chain, side view.
PDB entry 3kfq coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Renko M, Turk D

Function and Biology Details

Reaction catalysed: 
The recombinant enzyme hydrolyzes proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130178 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cathepsin L2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 24.09 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O60911 (Residues: 114-334; Coverage: 70%)
Gene names: CATL2, CTSL2, CTSU, CTSV, UNQ268/PRO305
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases
Cystatin-A Chains: C, D
Molecule details ›
Chains: C, D
Length: 98 amino acids
Theoretical weight: 11.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01040 (Residues: 1-98; Coverage: 100%)
Gene names: CSTA, STF1, STFA
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SCH 2 x SCH

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 59.313Å b: 107.29Å c: 115.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.176 0.219
Expression systems:
  • Komagataella pastoris
  • Escherichia coli BL21(DE3)

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