3kjm

X-ray diffraction
1.9Å resolution

Leu492Ala mutant of Maize cytokinin oxidase/dehydrogenase complexed with phenylurea inhibitor CPPU

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-193569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cytokinin dehydrogenase 1 Chain: A
Molecule details ›
Chain: A
Length: 516 amino acids
Theoretical weight: 55.32 KDa
Source organism: Zea mays
Expression system: Yarrowia lipolytica CLIB122
UniProt:
  • Canonical: Q9T0N8 (Residues: 19-534; Coverage: 100%)
Gene name: CKX1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
Carbohydrate polymer : NEW Components: NAG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 248.96Å b: 50.46Å c: 50.77Å
α: 90° β: 93.66° γ: 90°
R-values:
R R work R free
0.218 0.217 0.255
Expression system: Yarrowia lipolytica CLIB122