› 3klo

X-ray diffraction
2.8Å resolution

Vibrio cholerae VpsT bound to c-di-GMP

Released:
DOI: 10.2210/pdb3klo/pdb
PDB entry 3klo coloured by chain, front view.
PDB entry 3klo coloured by chain, side view.
PDB entry 3klo coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor
Primary publication:
Vibrio cholerae VpsT regulates matrix production and motility by directly sensing cyclic di-GMP.
Krasteva PV, Fong JC, Shikuma NJ, Beyhan S, Navarro MV, Yildiz FH, Sondermann H
Science 327 866-8 (2010)
PMID: 20150502

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191925 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH luxR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 225 amino acids
Theoretical weight: 25.97 KDa
Source organism: Vibrio cholerae O1 biovar El Tor
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KKZ8 (Residues: 1-224; Coverage: 100%)
Gene name: VC_A0952
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand C2E 4 x C2E
Ligand TAR 2 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 121.703Å b: 121.703Å c: 208.227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.246 0.29
Expression system: Escherichia coli BL21

Quick links

Citations

55 review citations

Bacterial quorum sensing: its role in virulence and possibilities for its control.
Rutherford et al. (2012)
54 more

11 mentions without citation

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
10 more