› 3kyt

X-ray diffraction
2.35Å resolution

Crystal structure of orphan nuclear receptor RORgamma in complex with natural ligand

Released:
DOI: 10.2210/pdb3kyt/pdb
PDB entry 3kyt coloured by chain, front view.
PDB entry 3kyt coloured by chain, side view.
PDB entry 3kyt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for hydroxycholesterols as natural ligands of orphan nuclear receptor RORgamma.
Jin L, Martynowski D, Zheng S, Wada T, Xie W, Li Y
Mol Endocrinol 24 923-9 (2010)
PMID: 20203100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156333 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear receptor ROR-gamma Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 28.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51449 (Residues: 265-507; Coverage: 47%)
Gene names: NR1F3, RORC, RORG, RZRG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 686-697; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand HC2 1 x HC2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P41212
Unit cell:
a: 53.978Å b: 53.978Å c: 162.583Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.197 0.298
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

37 review citations

25-Hydroxycholesterols in innate and adaptive immunity.
Cyster et al. (2014)
36 more

13 mentions without citation

Emerging models for the molecular basis of mammalian circadian timing.
Gustafson et al. (2015)
12 more