› 3l01

X-ray diffraction
2.6Å resolution

Crystal structure of monomeric glycogen synthase from Pyrococcus abyssi

Released:
DOI: 10.2210/pdb3l01/pdb
PDB entry 3l01 coloured by chain, front view.
PDB entry 3l01 coloured by chain, side view.
PDB entry 3l01 coloured by chain, top view.
Source organism: Pyrococcus abyssi
Primary publication:
Processivity and subcellular localization of glycogen synthase depend on a non-catalytic high affinity glycogen-binding site.
Díaz A, Martínez-Pons C, Fita I, Ferrer JC, Guinovart JJ
J Biol Chem 286 18505-14 (2011)
PMID: 21464127

Function and Biology Details

Reaction catalysed: 
ADP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = ADP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Starch synthase catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 48.08 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9V2J8 (Residues: 1-426; Coverage: 98%)
Gene name: PAB2292
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand K 6 x K
Ligand CL 6 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 103.604Å b: 119.017Å c: 141.101Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.228
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Glycogen and its metabolism: some new developments and old themes.
Roach et al. (2012)
4 more

2 mentions without citation

Processivity and subcellular localization of glycogen synthase depend on a non-catalytic high affinity glycogen-binding site.
Díaz et al. (2011)
1 more