› 3l4q

X-ray diffraction
2.3Å resolution

Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein

Released:
DOI: 10.2210/pdb3l4q/pdb
PDB entry 3l4q coloured by chain, front view.
PDB entry 3l4q coloured by chain, side view.
PDB entry 3l4q coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein.
Hale BG, Kerry PS, Jackson D, Precious BL, Gray A, Killip MJ, Randall RE, Russell RJ
Proc Natl Acad Sci U S A 107 1954-9 (2010)
PMID: 20133840

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137076 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.46 KDa
Source organism: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: P03496 (Residues: 73-230; Coverage: 69%)
Gene name: NS
Sequence domains: Influenza non-structural protein (NS1)
Structure domains: Influenza virus non-structural protein, effector domain
Phosphatidylinositol 3-kinase regulatory subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 170 amino acids
Theoretical weight: 20.63 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23726 (Residues: 424-593; Coverage: 24%)
Gene name: PIK3R2
Sequence domains: Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 57.95Å b: 98.67Å c: 149.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.292
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Structures of influenza A proteins and insights into antiviral drug targets.
Das et al. (2010)
15 more

11 mentions without citation

Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein.
Hale et al. (2010)
10 more