› 3l57

X-ray diffraction
2.29Å resolution

Crystal Structure of the Plasmid pCU1 TraI Relaxase Domain

Released:
DOI: 10.2210/pdb3l57/pdb
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PDB entry 3l57 coloured by chain, front view.
PDB entry 3l57 coloured by chain, side view.
PDB entry 3l57 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The mechanism and control of DNA transfer by the conjugative relaxase of resistance plasmid pCU1.
Nash RP, Habibi S, Cheng Y, Lujan SA, Redinbo MR
OpenAccess logo Nucleic Acids Res 38 5929-43 (2010)
PMID: 20448025

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mobilization protein TraI Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 33.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X4G2 (Residues: 1-299; Coverage: 28%)
Gene names: RCS42_P0021, RCS44_P0020, RCS45_P0020, pMUR050_047, traI
Sequence domains: TrwC relaxase

Ligands and Environments

4 bound ligands:
Ligand MN3 2 x MN3
Ligand EDO 26 x EDO
Ligand PO4 1 x PO4
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 50.28Å b: 58.52Å c: 87.12Å
α: 90° β: 83.88° γ: 90°
R-values:
R R work R free
0.176 0.173 0.226
Expression system: Escherichia coli

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Citations

2 review citations

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.
Chandler et al. (2013)
1 more

3 mentions without citation

The mechanism and control of DNA transfer by the conjugative relaxase of resistance plasmid pCU1.
Nash et al. (2010)
2 more

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