› 3l8w

X-ray diffraction
1Å resolution

Urate oxidase from aspergillus flavus complexed with xanthin

Released:
DOI: 10.2210/pdb3l8w/pdb
PDB entry 3l8w coloured by chain, front view.
PDB entry 3l8w coloured by chain, side view.
PDB entry 3l8w coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Near-atomic resolution structures of urate oxidase complexed with its substrate and analogues: the protonation state of the ligand.
Gabison L, Chiadmi M, El Hajji M, Castro B, Colloc'h N, Prangé T
Acta Crystallogr D Biol Crystallogr 66 714-24 (2010)
PMID: 20516624

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.52 KDa
Source organism: Aspergillus flavus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00511 (Residues: 2-296; Coverage: 98%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

3 bound ligands:
Ligand XAN 1 x XAN
Ligand NA 1 x NA
Ligand 4PO 1 x 4PO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I222
Unit cell:
a: 79.527Å b: 94.98Å c: 104.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.121 0.144
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

Mechanisms of nickel toxicity in microorganisms.
Macomber et al. (2011)
1 more

4 mentions without citation

AquaSAXS: a web server for computation and fitting of SAXS profiles with non-uniformally hydrated atomic models.
Poitevin et al. (2011)
3 more