› 3lc9

X-ray diffraction
2.28Å resolution

Ricin A-chain variant 1-33/44-198 with engineered disulfide bond

Released:
DOI: 10.2210/pdb3lc9/pdb
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PDB entry 3lc9 coloured by chain, front view.
PDB entry 3lc9 coloured by chain, side view.
PDB entry 3lc9 coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Introduction of a disulfide bond leads to stabilization and crystallization of a ricin immunogen.
Compton JR, Legler PM, Clingan BV, Olson MA, Millard CB
Proteins 79 1048-60 (2011)
PMID: 21387408

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.42 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02879 (Residues: 36-233; Coverage: 35%)
Sequence domains: Ribosome inactivating protein
Structure domains: Ricin (A subunit), domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: I222
Unit cell:
a: 51.493Å b: 72.307Å c: 94.208Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Immunity to ricin: fundamental insights into toxin-antibody interactions.
O'Hara et al. (2012)
2 more

4 mentions without citation

Structure of RiVax: a recombinant ricin vaccine.
Legler et al. (2011)
3 more

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