› 3ld0

X-ray diffraction
2.2Å resolution

Crystal structure of B.licheniformis Anti-TRAP protein, an antagonist of TRAP-RNA interactions

Released:
DOI: 10.2210/pdb3ld0/pdb
PDB entry 3ld0 coloured by chain, front view.
PDB entry 3ld0 coloured by chain, side view.
PDB entry 3ld0 coloured by chain, top view.
Source organism: Bacillus licheniformis DSM 13 = ATCC 14580
Primary publication:
Bacillus licheniformis Anti-TRAP can assemble into two types of dodecameric particles with the same symmetry but inverted orientation of trimers.
Shevtsov MB, Chen Y, Isupov MN, Leech A, Gollnick P, Antson AA
J Struct Biol 170 127-33 (2010)
PMID: 20138150

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inhibitor of TRAP, regulated by T-BOX (Trp) sequence RtpA Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m
Length: 53 amino acids
Theoretical weight: 5.74 KDa
Source organism: Bacillus licheniformis DSM 13 = ATCC 14580
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q65NU7 (Residues: 1-53; Coverage: 100%)
Gene names: BL05022, rtpA
Sequence domains: Tryptophan RNA-binding attenuator protein inhibitory protein
Structure domains: Chaperone, DNAj Protein; Chain A

Ligands and Environments

2 bound ligands:
Ligand ZN 48 x ZN
Ligand MG 16 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 118.544Å b: 99.865Å c: 123.178Å
α: 90° β: 117.61° γ: 90°
R-values:
R R work R free
0.194 0.194 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Space-group and origin ambiguity in macromolecular structures with pseudo-symmetry and its treatment with the program Zanuda.
Lebedev et al. (2014)
1 more

1 mention without citation

Bacillus licheniformis Anti-TRAP can assemble into two types of dodecameric particles with the same symmetry but inverted orientation of trimers.
Shevtsov et al. (2010)