› 3lf2

X-ray diffraction
2.3Å resolution

NADPH Bound Structure of the Short Chain Oxidoreductase Q9HYA2 from Pseudomonas aeruginosa PAO1 Containing an Atypical Catalytic Center

Released:
DOI: 10.2210/pdb3lf2/pdb
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Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
The short-chain oxidoreductase Q9HYA2 from Pseudomonas aeruginosa PAO1 contains an atypical catalytic center.
Huether R, Mao Q, Duax WL, Umland TC
Protein Sci 19 1097-103 (2010)
PMID: 20340135

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable short-chain dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 28.52 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HYA2 (Residues: 1-265; Coverage: 100%)
Gene name: PA3507
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

4 bound ligands:
Ligand NDP 2 x NDP
Ligand CL 3 x CL
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 65.788Å b: 112.607Å c: 136.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.238
Expression system: Escherichia coli

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Citations

1 citations in other articles

SDR7-6, a short-chain alcohol dehydrogenase/reductase family protein, regulates light-dependent cell death and defence responses in rice.
Zheng et al. (2022)

1 mention without citation

The short-chain oxidoreductase Q9HYA2 from Pseudomonas aeruginosa PAO1 contains an atypical catalytic center.
Huether et al. (2010)

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