› 3lgs

X-ray diffraction
2.2Å resolution

A. thaliana MTA nucleosidase in complex with S-adenosylhomocysteine

Released:
DOI: 10.2210/pdb3lgs/pdb
PDB entry 3lgs coloured by chain, front view.
PDB entry 3lgs coloured by chain, side view.
PDB entry 3lgs coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mechanism of substrate specificity in 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidases.
Siu KK, Asmus K, Zhang AN, Horvatin C, Li S, Liu T, Moffatt B, Woods VL, Howell PL
J Struct Biol 173 86-98 (2011)
PMID: 20554051

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + H(2)O = 5-(methylsulfanyl)-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine nucleosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.48 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9T0I8 (Residues: 1-267; Coverage: 100%)
Gene names: At4g38800, MTAN, MTAN1, MTN1, T9A14.80
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand SAH 4 x SAH
Ligand ADE 4 x ADE
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 45.85Å b: 126.41Å c: 83.81Å
α: 90° β: 101.73° γ: 90°
R-values:
R R work R free
0.21 0.21 0.26
Expression system: Escherichia coli BL21

Quick links

Citations

11 citation in other articles

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Hayden et al. (2011)
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2 mentions without citation

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