› 3lr9

X-ray diffraction
1.55Å resolution

X-ray photogenerated ferrous horse heart myoglobin, nitrite adduct

Released:
DOI: 10.2210/pdb3lr9/pdb
PDB entry 3lr9 coloured by chain, front view.
PDB entry 3lr9 coloured by chain, side view.
PDB entry 3lr9 coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Synchrotron X-ray-induced photoreduction of ferric myoglobin nitrite crystals gives the ferrous derivative with retention of the O-bonded nitrite ligand.
Yi J, Orville AM, Skinner JM, Skinner MJ, Richter-Addo GB
Biochemistry 49 5969-71 (2010)
PMID: 20568729

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-549791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.98 KDa
Source organism: Equus caballus
UniProt:
  • Canonical: P68082 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand NO2 4 x NO2
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 35.327Å b: 28.691Å c: 62.911Å
α: 90° β: 105.64° γ: 90°
R-values:
R R work R free
0.18 0.178 0.212

Quick links

Citations

1 review citation

Nitrite and nitrite reductases: from molecular mechanisms to significance in human health and disease.
Castiglione et al. (2012)
26 other articles

2 mentions without citation

Synchrotron X-ray-induced photoreduction of ferric myoglobin nitrite crystals gives the ferrous derivative with retention of the O-bonded nitrite ligand.
Yi et al. (2010)
1 more