› 3m0t

X-ray diffraction
1.7Å resolution

Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Crystal obtained in ammonium sulphate at pH 9.

Released:
DOI: 10.2210/pdb3m0t/pdb
PDB entry 3m0t coloured by chain, front view.
PDB entry 3m0t coloured by chain, side view.
PDB entry 3m0t coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Understanding the polymorphic behaviour of a mutant of the alpha-spectrin SH3 domain by means of two 1.1 A structures
Camara-Artigas A, Gavira JA, Casares S, Garcia-Ruiz JM, Conejero-Lara F, Allen JP, Martinez J
Acta Crystallogr. D Biol. Crystallogr. (2011)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.19 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07751 (Residues: 965-1025; Coverage: 3%)
Gene names: SPTA2, SPTAN1
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 34.621Å b: 42.211Å c: 49.104Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.215
Expression system: Escherichia coli

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