› 3m4i

X-ray diffraction
1.95Å resolution

Crystal structure of the second part of the Mycobacterium tuberculosis DNA gyrase reaction core: the TOPRIM domain at 1.95 A resolution

Released:
DOI: 10.2210/pdb3m4i/pdb
PDB entry 3m4i coloured by chain, front view.
PDB entry 3m4i coloured by chain, side view.
PDB entry 3m4i coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural insights into the quinolone resistance mechanism of Mycobacterium tuberculosis DNA gyrase.
Piton J, Petrella S, Delarue M, André-Leroux G, Jarlier V, Aubry A, Mayer C
OpenAccess logo PLoS One 5 e12245 (2010)
PMID: 20805881

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.42 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WG45 (Residues: 448-675; Coverage: 34%)
Gene names: MTCY10H4.03, Rv0005, gyrB
Sequence domains:
Structure domains: Dna Ligase; domain 1

Ligands and Environments

1 bound ligand:
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 52.815Å b: 52.815Å c: 190.462Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.23
Expression system: Escherichia coli

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Citations

13 review citations

Antimicrobial susceptibility testing, drug resistance mechanisms, and therapy of infections with nontuberculous mycobacteria.
Brown-Elliott et al. (2012)
12 more

4 mentions without citation

New insights into fluoroquinolone resistance in Mycobacterium tuberculosis: functional genetic analysis of gyrA and gyrB mutations.
Malik et al. (2012)
3 more