› 3mbp

X-ray diffraction
1.7Å resolution

MALTODEXTRIN-BINDING PROTEIN WITH BOUND MALTOTRIOSE

Released:
DOI: 10.2210/pdb3mbp/pdb
PDB entry 3mbp coloured by chain, front view.
PDB entry 3mbp coloured by chain, side view.
PDB entry 3mbp coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor.
Quiocho FA, Spurlino JC, Rodseth LE
Structure 5 997-1015 (1997)
PMID: 9309217

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 40.75 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 106.07Å b: 68.43Å c: 57.94Å
α: 90° β: 112.51° γ: 90°
R-values:
R R work R free
0.164 0.164 not available
Expression system: Not provided

Quick links

Citations

17 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
16 more

31 mentions without citation

Interplay between conformational selection and induced fit in multidomain protein-ligand binding probed by paramagnetic relaxation enhancement.
Clore GM. (2014)
30 more