› 3mey

X-ray diffraction
2.5Å resolution

Crystal structure of class II aaRS homologue (Bll0957) complexed with ATP

Released:
DOI: 10.2210/pdb3mey/pdb
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PDB entry 3mey coloured by chain, front view.
PDB entry 3mey coloured by chain, side view.
PDB entry 3mey coloured by chain, top view.
Source organism: Bradyrhizobium diazoefficiens USDA 110
Primary publication:
Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis.
Mocibob M, Ivic N, Bilokapic S, Maier T, Luic M, Ban N, Weygand-Durasevic I
Proc Natl Acad Sci U S A 107 14585-90 (2010)
PMID: 20663952

Function and Biology Details

Reaction catalysed: 
ATP + an amino acid + [acyl-carrier-protein] = AMP + diphosphate + an aminoacyl-[acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amino acid--[acyl-carrier-protein] ligase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 38.16 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q89VT8 (Residues: 1-326; Coverage: 100%)
Gene name: bll0957
Sequence domains: tRNA synthetase class II core domain (G, H, P, S and T)
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P21212
Unit cell:
a: 127.166Å b: 101.133Å c: 50.414Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.231
Expression system: Escherichia coli

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Citations

11 review citations

Nonproteinogenic amino acid building blocks for nonribosomal peptide and hybrid polyketide scaffolds.
Walsh et al. (2013)
10 more

4 mentions without citation

Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis.
Mocibob et al. (2010)
3 more

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