› 3mmk

X-ray diffraction
2.16Å resolution

The structural basis for partial redundancy in a class of transcription factors, the lim-homeodomain proteins, in neural cell type specification

Released:
DOI: 10.2210/pdb3mmk/pdb
PDB entry 3mmk coloured by chain, front view.
PDB entry 3mmk coloured by chain, side view.
PDB entry 3mmk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for partial redundancy in a class of transcription factors, the LIM homeodomain proteins, in neural cell type specification.
Gadd MS, Bhati M, Jeffries CM, Langley DB, Trewhella J, Guss JM, Matthews JM
J Biol Chem 286 42971-80 (2011)
PMID: 22025611

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin gene enhancer protein ISL-2; LIM/homeobox protein Lhx4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P53776 (Residues: 24-149; Coverage: 32%)
  • Canonical: Q9CXV0 (Residues: 149-166; Coverage: 5%)
Gene names: Gsh-4, Gsh4, Isl2, Lhx4
Sequence domains: LIM domain
Structure domains: Cysteine Rich Protein

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 46.823Å b: 88.734Å c: 49.851Å
α: 90° β: 111.85° γ: 90°
R-values:
R R work R free
0.237 0.235 0.265
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Selecting optimal combinations of transcription factors to promote axon regeneration: Why mechanisms matter.
Venkatesh et al. (2017)
1 more

5 mentions without citation

Linkers in the structural biology of protein-protein interactions.
Reddy Chichili et al. (2013)
4 more