› 3mmw

X-ray diffraction
1.85Å resolution

Crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima

Released:
DOI: 10.2210/pdb3mmw/pdb
PDB entry 3mmw coloured by chain, front view.
PDB entry 3mmw coloured by chain, side view.
PDB entry 3mmw coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima.
Pereira JH, Chen Z, McAndrew RP, Sapra R, Chhabra SR, Sale KL, Simmons BA, Adams PD
J Struct Biol 172 372-9 (2010)
PMID: 20599513

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-194874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 5 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 37.44 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X273 (Residues: 1-317; Coverage: 100%)
Gene name: TM_1751
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand CD 11 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 85.814Å b: 81.407Å c: 103.139Å
α: 90° β: 114.43° γ: 90°
R-values:
R R work R free
0.19 0.189 0.217
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nature versus nurture: developing enzymes that function under extreme conditions.
Liszka et al. (2012)
3 more

5 mentions without citation

High resolution crystal structure of human β-glucuronidase reveals structural basis of lysosome targeting.
Hassan et al. (2013)
4 more