› 3moe

X-ray diffraction
1.25Å resolution

The structure of rat cytosolic PEPCK mutant A467G in complex with Beta-Sulfopyruvate and GTP

Released:
DOI: 10.2210/pdb3moe/pdb
PDB entry 3moe coloured by chain, front view.
PDB entry 3moe coloured by chain, side view.
PDB entry 3moe coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Increasing the conformational entropy of the Omega-loop lid domain in phosphoenolpyruvate carboxykinase impairs catalysis and decreases catalytic fidelity .
Johnson TA, Holyoak T
Biochemistry 49 5176-87 (2010)
PMID: 20476774

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Chain: A
Molecule details ›
Chain: A
Length: 624 amino acids
Theoretical weight: 69.63 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07379 (Residues: 1-622; Coverage: 100%)
Gene name: Pck1
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand GTP 1 x GTP
Ligand MN 2 x MN
Ligand NA 1 x NA
Ligand SPV 1 x SPV
Ligand 1PE 3 x 1PE
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 44.324Å b: 119.117Å c: 60.053Å
α: 90° β: 111.19° γ: 90°
R-values:
R R work R free
0.149 0.148 0.174
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The mitochondrial isoform of phosphoenolpyruvate carboxykinase (PEPCK-M) and glucose homeostasis: has it been overlooked?
Stark et al. (2014)
8 other articles

2 mentions without citation

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
1 more