› 3mpb

X-ray diffraction
1.91Å resolution

Z5688 from E. coli O157:H7 bound to fructose

Released:
DOI: 10.2210/pdb3mpb/pdb
PDB entry 3mpb coloured by chain, front view.
PDB entry 3mpb coloured by chain, side view.
PDB entry 3mpb coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Structure-based annotation of a novel sugar isomerase from the pathogenic E. coli O157:H7.
van Staalduinen LM, Park CS, Yeom SJ, Adams-Cioaba MA, Oh DK, Jia Z
J Mol Biol 401 866-81 (2010)
PMID: 20615418

Function and Biology Details

Reaction catalysed: 
D-lyxose = D-xylulose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-570795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-lyxose ketol-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.86 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X5Q7 (Residues: 1-227; Coverage: 100%)
Gene name: ECs_5070
Sequence domains: D-lyxose isomerase
Structure domains: Jelly Rolls

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand ACT 2 x ACT
Ligand FRU 2 x FRU
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 53.425Å b: 75.699Å c: 62.022Å
α: 90° β: 106.54° γ: 90°
R-values:
R R work R free
0.191 0.188 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

D-lyxose isomerase and its application for functional sugar production.
Huang et al. (2018)
4 more