› 3msv

X-ray diffraction
2.18Å resolution

The hypoxic regulator of sterol synthesis Nro1 is a nuclear import adaptor

Released:
DOI: 10.2210/pdb3msv/pdb
PDB entry 3msv coloured by chain, front view.
PDB entry 3msv coloured by chain, side view.
PDB entry 3msv coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
The hypoxic regulator of sterol synthesis nro1 is a nuclear import adaptor.
Yeh TL, Lee CY, Amzel LM, Espenshade PJ, Bianchet MA
Structure 19 503-14 (2011)
PMID: 21481773

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-194088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Negative regulator of ofd1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 44.49 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9USJ7 (Residues: 1-393; Coverage: 100%)
Gene names: SPCC4B3.07, nro1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT, APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 47.79Å b: 107.96Å c: 100.78Å
α: 90° β: 102.6° γ: 90°
R-values:
R R work R free
0.207 0.205 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Hypoxia and gene expression in eukaryotic microbes.
Butler G. (2013)
1 more

1 mention without citation

The hypoxic regulator of sterol synthesis nro1 is a nuclear import adaptor.
Yeh et al. (2011)