› 3mti

X-ray diffraction
1.95Å resolution

The Crystal Structure of a rRNA Methylase from Streptococcus thermophilus to 1.95A

Released:
DOI: 10.2210/pdb3mti/pdb
PDB entry 3mti coloured by chain, front view.
PDB entry 3mti coloured by chain, side view.
PDB entry 3mti coloured by chain, top view.
Source organism: Streptococcus thermophilus LMG 18311
Entry authors: Stein AJ, Mulligan R, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA methylase (SAM-dependent methyltransferase superfamily), putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 21 KDa
Source organism: Streptococcus thermophilus LMG 18311
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5M2Z6 (Residues: 1-182; Coverage: 100%)
Gene name: stu1645
Sequence domains: Putative rRNA methylase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 48.331Å b: 57.044Å c: 75.365Å
α: 90° β: 109.11° γ: 90°
R-values:
R R work R free
0.24 0.238 0.272
Expression system: Escherichia coli BL21(DE3)

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