› 3mx6

X-ray diffraction
1.7Å resolution

Crystal structure of methionine aminopeptidase from Rickettsia prowazekii bound to methionine

Released:
DOI: 10.2210/pdb3mx6/pdb
PDB entry 3mx6 coloured by chain, front view.
PDB entry 3mx6 coloured by chain, side view.
PDB entry 3mx6 coloured by chain, top view.
Source organism: Rickettsia prowazekii
Primary publication:
Rickettsia prowazekii methionine aminopeptidase as a promising target for the development of antibacterial agents.
Helgren TR, Chen C, Wangtrakuldee P, Edwards TE, Staker BL, Abendroth J, Sankaran B, Housley NA, Myler PJ, Audia JP, Horn JR, Hagen TJ
Bioorg Med Chem 25 813-824 (2017)
PMID: 28089350

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-579198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.1 KDa
Source organism: Rickettsia prowazekii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZCD3 (Residues: 3-259; Coverage: 99%)
Gene names: RP824, map
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

3 bound ligands:
Ligand MET 2 x MET
Ligand MN 4 x MN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 50.37Å b: 67.55Å c: 80.89Å
α: 90° β: 97.43° γ: 90°
R-values:
R R work R free
0.169 0.168 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Synthesis of Bioactive Aminomethylated 8-Hydroxyquinolines via the Modified Mannich Reaction.
Csuvik et al. (2023)
3 other articles

3 mentions without citation

P1' Residue-Oriented Virtual Screening for Potent and Selective Phosphinic (Dehydro) Dipeptide Inhibitors of Metallo-Aminopeptidases.
Talma et al. (2020)
2 more