› 3n2j

X-ray diffraction
1.35Å resolution

Azurin H117G, oxidized form

Released:
DOI: 10.2210/pdb3n2j/pdb
PDB entry 3n2j coloured by chain, front view.
PDB entry 3n2j coloured by chain, side view.
PDB entry 3n2j coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Probing the reactivity of different forms of azurin by flavin photoreduction.
Alagaratnam S, Meeuwenoord NJ, Navarro JA, Hervás M, De la Rosa MA, Hoffmann M, Einsle O, Ubbink M, Canters GW
FEBS J 278 1506-21 (2011)
PMID: 21352498

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 128 amino acids
Theoretical weight: 13.88 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 24 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21
Unit cell:
a: 47.545Å b: 170.008Å c: 85.737Å
α: 90° β: 89.98° γ: 90°
R-values:
R R work R free
0.174 0.172 0.202
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
1 more

1 mention without citation

Probing the reactivity of different forms of azurin by flavin photoreduction.
Alagaratnam et al. (2011)