› 3nb9

X-ray diffraction
1.5Å resolution

Crystal structure of radical H88Q Synechocystis sp. PCYA

Released:
DOI: 10.2210/pdb3nb9/pdb
PDB entry 3nb9 coloured by chain, front view.
PDB entry 3nb9 coloured by chain, side view.
PDB entry 3nb9 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Structural basis for hydration dynamics in radical stabilization of bilin reductase mutants.
Kohler AC, Gae DD, Richley MA, Stoll S, Gunn A, Lim S, Martin SS, Doukov TI, Britt RD, Ames JB, Lagarias JC, Fisher AJ
Biochemistry 49 6206-18 (2010)
PMID: 20557110

Function and Biology Details

Reaction catalysed: 
(3Z)-phycocyanobilin + 4 oxidized ferredoxin = biliverdin IX-alpha + 4 reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phycocyanobilin:ferredoxin oxidoreductase Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.15 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55891 (Residues: 1-248; Coverage: 100%)
Gene names: pcyA, slr0116
Sequence domains: Ferredoxin-dependent bilin reductase
Structure domains: oxygen-dependent coproporphyrinogen oxidase

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand BLA 1 x BLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 70.84Å b: 95.229Å c: 42.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.171 0.196
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Plant type ferredoxins and ferredoxin-dependent metabolism.
Hanke et al. (2013)
8 other articles

1 mention without citation

Structural basis for hydration dynamics in radical stabilization of bilin reductase mutants.
Kohler et al. (2010)