3ngz › Citations

Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation.

Hsiao YY, Yang CC, Lin CL, Lin JL, Duh Y, Yuan HS
Nat Chem Biol 7 236-43 (2011)
Related entries: 3ngy, 3nh0, 3nh1, 3nh2

Cited: 23 times
EuropePMC logo PMID: 21317904

Abstract

RNA maturation relies on various exonucleases to remove nucleotides successively from the 5' or 3' end of nucleic acids. However, little is known regarding the molecular basis for substrate and cleavage preference of exonucleases. Our biochemical and structural analyses on RNase T-DNA complexes show that the RNase T dimer has an ideal architecture for binding a duplex with a short 3' overhang to produce a digestion product of a duplex with a 2-nucleotide (nt) or 1-nt 3' overhang, depending on the composition of the last base pair in the duplex. A 'C-filter' in RNase T screens out the nucleic acids with 3'-terminal cytosines for hydrolysis by inducing a disruptive conformational change at the active site. Our results reveal the general principles and the working mechanism for the final trimming step made by RNase T in the maturation of stable RNA and pave the way for the understanding of other DEDD family exonucleases.

Reviews - 3ngz mentioned but not cited (1)

  1. Not making the cut: Techniques to prevent RNA cleavage in structural studies of RNase-RNA complexes. Jones SP, Goossen C, Lewis SD, Delaney AM, Gleghorn ML. J Struct Biol X 6 100066 (2022)

Articles - 3ngz mentioned but not cited (1)



Reviews citing this publication (3)

  1. Bacterial ribonucleases and their roles in RNA metabolism. Bechhofer DH, Deutscher MP. Crit Rev Biochem Mol Biol 54 242-300 (2019)
  2. Understanding APE1 cellular functions by the structural preference of exonuclease activities. Liu TC, Guo KW, Chu JW, Hsiao YY. Comput Struct Biotechnol J 19 3682-3691 (2021)
  3. Nano-RNases: oligo- or dinucleases? Lee VT, Sondermann H, Winkler WC. FEMS Microbiol Rev 46 fuac038 (2022)

Articles citing this publication (18)



Quick links