› 3nhq

X-ray diffraction
2.55Å resolution

The dark Pfr structure of the photosensory core module of P. aeruginosa Bacteriophytochrome

Released:
DOI: 10.2210/pdb3nhq/pdb
PDB entry 3nhq coloured by chain, front view.
PDB entry 3nhq coloured by chain, side view.
PDB entry 3nhq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome.
Yang X, Ren Z, Kuk J, Moffat K
Nature 479 428-32 (2011)
PMID: 22002602

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 505 amino acids
Theoretical weight: 56.82 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HWR3 (Residues: 1-497; Coverage: 68%)
Gene names: PA4117, bphP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BLA 8 x BLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2221
Unit cell:
a: 154.38Å b: 162.979Å c: 436.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.258
Expression system: Escherichia coli BL21

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Citations

7 review citations

Phytochromes: an atomic perspective on photoactivation and signaling.
Burgie et al. (2014)
6 more

11 mentions without citation

Bacteriophytochromes - from informative model systems of phytochrome function to powerful tools in cell biology.
Gourinchas et al. (2019)
10 more