› 3nmi

X-ray diffraction
2.01Å resolution

Crystal structure of the phenanthroline-modified cytochrome cb562 variant, MBP-Phen2

Released:
DOI: 10.2210/pdb3nmi/pdb
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PDB entry 3nmi coloured by chain, front view.
PDB entry 3nmi coloured by chain, side view.
PDB entry 3nmi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Porous protein frameworks with unsaturated metal centers in sterically encumbered coordination sites.
Radford RJ, Lawrenz M, Nguyen PC, McCammon JA, Tezcan FA
Chem Commun (Camb) 47 313-5 (2011)
PMID: 20740227

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 106 amino acids
Theoretical weight: 11.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

3 bound ligands:
Ligand HEM 6 x HEM
Ligand PXX 6 x PXX
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 88.29Å b: 91.787Å c: 130.637Å
α: 90° β: 107.99° γ: 90°
R-values:
R R work R free
0.2 0.198 0.235
Expression system: Escherichia coli

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Citations

2 review citations

Metal-biomolecule frameworks (MBioFs).
Imaz et al. (2011)
1 more

1 mention without citation

Significant reduction in errors associated with nonbonded contacts in protein crystal structures: automated all-atom refinement with PrimeX.
Bell et al. (2012)

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