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Crystal Structure of CUGBP1 RRM1/2-RNA Complex

X-ray diffraction
1.899Å resolution
PDB entry 3nna coloured by chain, front view.
PDB entry 3nna coloured by chain, side view.
PDB entry 3nna coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CUGBP Elav-like family member 1 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 10991.47 Å2
Buried surface area: 1115.29 Å2
Dissociation area: 557.64 Å2
Dissociation energy (ΔGdiss): 8.8 kcal/mol
Dissociation entropy (TΔSdiss): 8.02 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-187794

Macromolecules

CUGBP Elav-like family member 1
Chain: A
Length: 175 amino acids
Theoretical weight: 20.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92879 (Residues: 14-187; Coverage: 36%)
Gene names: BRUNOL2, CELF1, CUGBP, CUGBP1, NAB50
Pfam: RNA recognition motif
InterPro:
CATH: Alpha-Beta Plaits
CUGBP Elav-like family member 1 in PDB entry 3nna, assembly 1, front view.
CUGBP Elav-like family member 1 in PDB entry 3nna, assembly 1, side view.
CUGBP Elav-like family member 1 in PDB entry 3nna, assembly 1, top view.
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RNA chain B
Name: RNA (5'-R(*GP*UP*UP*GP*UP*UP*UP*UP*GP*UP*UP*U)-3')
Representative chains: B
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.75 KDa
RNA (5'-R(*GP*UP*UP*GP*UP*UP*UP*UP*GP*UP*UP*U)-3') in PDB entry 3nna, assembly 1, front view.
RNA (5'-R(*GP*UP*UP*GP*UP*UP*UP*UP*GP*UP*UP*U)-3') in PDB entry 3nna, assembly 1, side view.
RNA (5'-R(*GP*UP*UP*GP*UP*UP*UP*UP*GP*UP*UP*U)-3') in PDB entry 3nna, assembly 1, top view.
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