› 3nnn

X-ray diffraction
2.2Å resolution

BeF3 Activated DrrD Receiver Domain

Released:
DOI: 10.2210/pdb3nnn/pdb
PDB entry 3nnn coloured by chain, front view.
PDB entry 3nnn coloured by chain, side view.
PDB entry 3nnn coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Regulation of response regulator autophosphorylation through interdomain contacts.
Barbieri CM, Mack TR, Robinson VL, Miller MT, Stock AM
OpenAccess logo J Biol Chem 285 32325-35 (2010)
PMID: 20702407

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-578185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.99 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WYN0 (Residues: 1-122; Coverage: 54%)
Gene name: TM_0399
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand BEF 2 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43212
Unit cell:
a: 74.951Å b: 74.951Å c: 98.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
5 more

5 mentions without citation

All DACs in a Row: Domain Architectures of Bacterial and Archaeal Diadenylate Cyclases.
Galperin MY. (2023)
4 more