› 3nu8

X-ray diffraction
1.5Å resolution

WbpE, an Aminotransferase from Pseudomonas aeruginosa Involved in O-antigen Assembly in Complex with the Internal Aldimine

Released:
DOI: 10.2210/pdb3nu8/pdb
PDB entry 3nu8 coloured by chain, front view.
PDB entry 3nu8 coloured by chain, side view.
PDB entry 3nu8 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural analysis of WbpE from Pseudomonas aeruginosa PAO1: a nucleotide sugar aminotransferase involved in O-antigen assembly.
Larkin A, Olivier NB, Imperiali B
Biochemistry 49 7227-37 (2010)
PMID: 20604544

Function and Biology Details

Reaction catalysed: 
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate = UDP-2-acetamido-2-deoxy-D-ribo-hex-3-uluronate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 39.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HZ76 (Residues: 1-359; Coverage: 100%)
Gene names: PA3155, wbpE
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21212
Unit cell:
a: 77.786Å b: 148.723Å c: 53.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.223
Expression system: Escherichia coli

Quick links

Citations

3 review citations

N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.
Jarrell et al. (2014)
2 more

2 mentions without citation

The structure of NtdA, a sugar aminotransferase involved in the kanosamine biosynthetic pathway in Bacillus subtilis, reveals a new subclass of aminotransferases.
van Straaten et al. (2013)
1 more