› 3nue

X-ray diffraction
2.5Å resolution

The structure of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase from mycobacterium tuberculosis complexed with tryptophan

Released:
DOI: 10.2210/pdb3nue/pdb
PDB entry 3nue coloured by chain, front view.
PDB entry 3nue coloured by chain, side view.
PDB entry 3nue coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Synergistic allostery, a sophisticated regulatory network for the control of aromatic amino acid biosynthesis in Mycobacterium tuberculosis.
Webby CJ, Jiao W, Hutton RD, Blackmore NJ, Baker HM, Baker EN, Jameson GB, Parker EJ
J Biol Chem 285 30567-76 (2010)
PMID: 20667835

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase AroG Chains: A, B
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 50.83 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: O53512 (Residues: 1-462; Coverage: 100%)
Gene names: Rv2178c, aroG
Sequence domains: Class-II DAHP synthetase family

Ligands and Environments

7 bound ligands:
Ligand MN 2 x MN
Ligand TRP 2 x TRP
Ligand PO4 2 x PO4
Ligand SO4 4 x SO4
Ligand GOL 7 x GOL
Ligand CE1 1 x CE1
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3221
Unit cell:
a: 204.712Å b: 204.712Å c: 66.264Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.228
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The shikimate pathway and aromatic amino Acid biosynthesis in plants.
Maeda et al. (2012)
5 more

2 mentions without citation

Synergistic allostery, a sophisticated regulatory network for the control of aromatic amino acid biosynthesis in Mycobacterium tuberculosis.
Webby et al. (2010)
1 more