› 3nzc

X-ray diffraction
2Å resolution

Structural Analysis of Pneumocystis carinii and Human DHFR Complexes with NADPH and a Series of Five Potent 5-(omega-carboxy(alkyloxy)pyrido[2,3-d]pyridine Derivativea

Released:
DOI: 10.2210/pdb3nzc/pdb
PDB entry 3nzc coloured by chain, front view.
PDB entry 3nzc coloured by chain, side view.
PDB entry 3nzc coloured by chain, top view.
Source organism: Pneumocystis carinii
Primary publication:
Structural analysis of Pneumocystis carinii and human DHFR complexes with NADPH and a series of five potent 6-[5'-(ω-carboxyalkoxy)benzyl]pyrido[2,3-d]pyrimidine derivatives.
Cody V, Pace J
Acta Crystallogr D Biol Crystallogr 67 1-7 (2011)
PMID: 21206056

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: X
Molecule details ›
Chain: X
Length: 206 amino acids
Theoretical weight: 23.92 KDa
Source organism: Pneumocystis carinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P16184 (Residues: 1-206; Coverage: 100%)
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

3 bound ligands:
Ligand D2O 1 x D2O
Ligand PO4 1 x PO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 36.859Å b: 42.667Å c: 59.936Å
α: 90° β: 94.77° γ: 90°
R-values:
R R work R free
0.212 0.209 0.26
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Utility of the Biosynthetic Folate Pathway for Targets in Antimicrobial Discovery.
Bourne CR. (2014)
1 more

3 mentions without citation

Antifolate agents: a patent review (2006 - 2010).
Wright et al. (2011)
2 more