› 3o4v

X-ray diffraction
1.75Å resolution

Crystal structure of E. coli MTA/SAH nucleosidase in complex with (4-Chlorophenyl)thio-DADMe-ImmA

Released:
DOI: 10.2210/pdb3o4v/pdb
PDB entry 3o4v coloured by chain, front view.
PDB entry 3o4v coloured by chain, side view.
PDB entry 3o4v coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Siu KKW, Howell PL

Function and Biology Details

Reaction catalysed: 
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 24.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AF12 (Residues: 1-232; Coverage: 100%)
Gene names: JW0155, b0159, mtn, mtnN, pfs, yadA
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand 4CT 2 x 4CT
Ligand GOL 4 x GOL
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 51.6Å b: 69.09Å c: 128.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.152 0.194
Expression system: Escherichia coli BL21

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