› 3obi

X-ray diffraction
1.95Å resolution

Crystal structure of a formyltetrahydrofolate deformylase (NP_949368) from RHODOPSEUDOMONAS PALUSTRIS CGA009 at 1.95 A resolution

Released:
DOI: 10.2210/pdb3obi/pdb
PDB entry 3obi coloured by chain, front view.
PDB entry 3obi coloured by chain, side view.
PDB entry 3obi coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyltetrahydrofolate deformylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 288 amino acids
Theoretical weight: 33.05 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6N2L8 (Residues: 1-287; Coverage: 100%)
Gene names: RPA4032, TX73_020890, purU
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 29 x EDO
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 70.048Å b: 141.111Å c: 77.563Å
α: 90° β: 107.83° γ: 90°
R-values:
R R work R free
0.236 0.234 0.278
Expression system: Escherichia coli

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