› 3of1

X-ray diffraction
2.21Å resolution

Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA

Released:
DOI: 10.2210/pdb3of1/pdb
PDB entry 3of1 coloured by chain, front view.
PDB entry 3of1 coloured by chain, side view.
PDB entry 3of1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling.
Rinaldi J, Wu J, Yang J, Ralston CY, Sankaran B, Moreno S, Taylor SS
Structure 18 1471-82 (2010)
PMID: 21070946

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase regulatory subunit Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P07278 (Residues: 171-416; Coverage: 59%)
Gene names: BCY1, REG1, SRA1, YIL033C
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 146.425Å b: 45.042Å c: 39.103Å
α: 90° β: 92.51° γ: 90°
R-values:
R R work R free
0.2 0.2 0.263
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Evolution of the eukaryotic protein kinases as dynamic molecular switches.
Taylor et al. (2012)
2 more

2 mentions without citation

Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling.
Rinaldi et al. (2010)
1 more