› 3ofm

X-ray diffraction
2Å resolution

Structure of a human CK2alpha prime, the paralog isoform of the catalytic subunit of protein kinase CK2 from Homo sapiens

Released:
DOI: 10.2210/pdb3ofm/pdb
PDB entry 3ofm coloured by chain, front view.
PDB entry 3ofm coloured by chain, side view.
PDB entry 3ofm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human protein kinase CK2 catalytic subunit CK2α' and interaction thermodynamics with the regulatory subunit CK2β.
Bischoff N, Olsen B, Raaf J, Bretner M, Issinger OG, Niefind K
J Mol Biol 407 1-12 (2011)
PMID: 21241709

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-539257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha' Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 41.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19784 (Residues: 1-350; Coverage: 100%)
Gene names: CK2A2, CSNK2A2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 4B0 1 x 4B0
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 46.69Å b: 47.57Å c: 50.57Å
α: 112.4° β: 89.49° γ: 91.3°
R-values:
R R work R free
0.174 0.172 0.207
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The New Role for an Old Kinase: Protein Kinase CK2 Regulates Metal Ion Transport.
Johnson et al. (2016)
2 more

7 mentions without citation

The Development of CK2 Inhibitors: From Traditional Pharmacology to in Silico Rational Drug Design.
Cozza G. (2017)
6 more