› 3og3

X-ray diffraction
2.08Å resolution

Crystal structure of an artificial thermostable (BA)8-barrel protein from identical half barrels

Released:
DOI: 10.2210/pdb3og3/pdb
PDB entry 3og3 coloured by chain, front view.
PDB entry 3og3 coloured by chain, side view.
PDB entry 3og3 coloured by chain, top view.
Source organism: Thermotoga maritima
Entry authors: Sperl JM, Bocola M, List F, Kellerer B, Sterner R

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazole glycerol phosphate synthase subunit HisF Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 27.32 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X0C6 (Residues: 4-122; Coverage: 84%)
Gene names: TM_1036, hisF
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: C2
Unit cell:
a: 31.374Å b: 82.086Å c: 74.511Å
α: 90° β: 90.11° γ: 90°
R-values:
R R work R free
0.166 0.15 0.196
Expression system: Escherichia coli

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