› 3oht

X-ray diffraction
2.7Å resolution

Crystal Structure of Salmo Salar p38alpha

Released:
DOI: 10.2210/pdb3oht/pdb
PDB entry 3oht coloured by chain, front view.
PDB entry 3oht coloured by chain, side view.
PDB entry 3oht coloured by chain, top view.
Source organism: Salmo salar
Primary publication:
p38α MAP kinase dimers with swapped activation segments and a novel catalytic loop conformation.
Rothweiler U, Åberg E, Johnson KA, Hansen TE, Jørgensen JB, Engh RA
J Mol Biol 411 474-85 (2011)
PMID: 21699901

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109047 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mitogen-activated protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 44.96 KDa
Source organism: Salmo salar
Expression system: Escherichia coli
UniProt:
  • Canonical: A9UJZ9 (Residues: 1-361; Coverage: 100%)
Gene name: p38a
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 1N1 4 x 1N1
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 67.78Å b: 100.93Å c: 67.89Å
α: 90° β: 98.15° γ: 90°
R-values:
R R work R free
0.235 0.232 0.289
Expression system: Escherichia coli

Quick links

Citations

1 review citation

How Do Protein Kinases Take a Selfie (Autophosphorylate)?
Beenstock et al. (2016)
11 other articles

2 mentions without citation

Conformational analysis of the DFG-out kinase motif and biochemical profiling of structurally validated type II inhibitors.
Vijayan et al. (2015)
1 more