› 3ok0

X-ray diffraction
1.82Å resolution

E35A Mutant of Hen Egg White Lysozyme (HEWL)

Released:
DOI: 10.2210/pdb3ok0/pdb
PDB entry 3ok0 coloured by chain, front view.
PDB entry 3ok0 coloured by chain, side view.
PDB entry 3ok0 coloured by chain, top view.
Source organism: Gallus gallus
Entry authors: O'Meara F, Bradley J, O'Rourke PE, Webb H, Tynan-Connolly BM, Nielsen JE

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.27 KDa
Source organism: Gallus gallus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43212
Unit cell:
a: 79.033Å b: 79.033Å c: 37.913Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.184
Expression system: Komagataella pastoris

Quick links