› 3oo6

X-ray diffraction
2.15Å resolution

Crystal structures and biochemical characterization of the bacterial solute receptor AcbH reveal an unprecedented exclusive substrate preference for b-D-galactopyranose

Released:
DOI: 10.2210/pdb3oo6/pdb
PDB entry 3oo6 coloured by chain, front view.
PDB entry 3oo6 coloured by chain, side view.
PDB entry 3oo6 coloured by chain, top view.
Source organism: Actinoplanes sp. SE50/110
Primary publication:
Crystal structures of the bacterial solute receptor AcbH displaying an exclusive substrate preference for β-D-galactopyranose.
Licht A, Bulut H, Scheffel F, Daumke O, Wehmeier UF, Saenger W, Schneider E, Vahedi-Faridi A
J Mol Biol 406 92-105 (2011)
PMID: 21168419

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter binding protein AcbH Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 45.87 KDa
Source organism: Actinoplanes sp. SE50/110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27GR2 (Residues: 39-433; Coverage: 98%)
Gene names: ACPL_3687, AcbH, acbH
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand GAL 2 x GAL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 47.835Å b: 98.812Å c: 95.732Å
α: 90° β: 95.04° γ: 90°
R-values:
R R work R free
0.191 0.188 0.251
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110.
Schwientek et al. (2012)
10 more

3 mentions without citation

Biochemical and structural insights into xylan utilization by the thermophilic bacterium Caldanaerobius polysaccharolyticus.
Han et al. (2012)
2 more