› 3otr

X-ray diffraction
2.75Å resolution

2.75 Angstrom Crystal Structure of Enolase 1 from Toxoplasma gondii

Released:
DOI: 10.2210/pdb3otr/pdb
PDB entry 3otr coloured by chain, front view.
PDB entry 3otr coloured by chain, side view.
PDB entry 3otr coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
The structure of bradyzoite-specific enolase from Toxoplasma gondii reveals insights into its dual cytoplasmic and nuclear functions.
Ruan J, Mouveaux T, Light SH, Minasov G, Anderson WF, Tomavo S, Ngô HM
OpenAccess logo Acta Crystallogr D Biol Crystallogr 71 417-26 (2015)
PMID: 25760592

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphopyruvate hydratase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 452 amino acids
Theoretical weight: 49.48 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli
UniProt:
  • Canonical: B9PH47 (Residues: 1-444; Coverage: 100%)
Gene names: BN1205_015870, TGVEG_268860
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I4
Unit cell:
a: 323.632Å b: 323.632Å c: 66.773Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.219
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Observations on bradyzoite biology.
Tu et al. (2018)
1 more

8 mentions without citation

The structure of bradyzoite-specific enolase from Toxoplasma gondii reveals insights into its dual cytoplasmic and nuclear functions.
Ruan et al. (2015)
7 more