Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
- not assigned
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo pentamer (preferred)
Assembly name:
Gag Polyprotein and Capsid protein p24 (preferred)
PDBe Complex ID:
PDB-CPX-146237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein p24
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 231 amino acids
Theoretical weight: 25.42 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 231 amino acids
Theoretical weight: 25.42 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
- Canonical:
P12497 (Residues: 133-363; Coverage: 16%) - Canonical: Q72497 (Residues: 133-363; Coverage: 46%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
APS BEAMLINE 22-ID
Spacegroup:
P1
Expression system: Escherichia coli
