› 3p4e

X-ray diffraction
1.77Å resolution

Phosphoribosylformylglycinamidine cyclo-ligase from Vibrio cholerae

Released:
DOI: 10.2210/pdb3p4e/pdb
PDB entry 3p4e coloured by chain, front view.
PDB entry 3p4e coloured by chain, side view.
PDB entry 3p4e coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Osipiuk J, Zhou M, Papazisi L, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192017 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylformylglycinamidine cyclo-ligase Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 37.08 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KPY6 (Residues: 1-346; Coverage: 100%)
Gene names: VC_2226, purM
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand AMP 1 x AMP
Ligand GOL 1 x GOL
Ligand CIT 1 x CIT
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 106.277Å b: 106.277Å c: 61.379Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.149 0.175
Expression system: Escherichia coli BL21(DE3)

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