› 3p8c

X-ray diffraction
2.29Å resolution

Structure and Control of the Actin Regulatory WAVE Complex

Released:
DOI: 10.2210/pdb3p8c/pdb
Model geometry
Fit model/data
PDB entry 3p8c coloured by chain, front view.
PDB entry 3p8c coloured by chain, side view.
PDB entry 3p8c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and control of the actin regulatory WAVE complex.
Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK
OpenAccess logo Nature 468 533-8 (2010)
PMID: 21107423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-181632 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cytoplasmic FMR1-interacting protein 1 Chain: A
Molecule details ›
Chain: A
Length: 1253 amino acids
Theoretical weight: 145.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7L576 (Residues: 1-1253; Coverage: 100%)
Gene names: CYFIP1, KIAA0068
Sequence domains:
Nck-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 1128 amino acids
Theoretical weight: 128.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2A7 (Residues: 1-1128; Coverage: 100%)
Gene names: HEM2, KIAA0587, NAP1, NCKAP1
Sequence domains: Nck-associated protein 1
Actin-binding protein WASF1 Chain: D
Protein BRICK1 Chain: E
Abl interactor 2 Chain: F

Ligands and Environments

3 bound ligands:
Ligand TRS 1 x TRS
Ligand CL 7 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 97.013Å b: 113.982Å c: 327.193Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.237
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

80 review citations

Life at the leading edge.
Ridley AJ. (2011)
79 more

32 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
31 more