› 3py9

X-ray diffraction
2.2Å resolution

X-ray structural studies of the entire extra-cellular region of the Ser/Thr kinase PrkC from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3py9/pdb
PDB entry 3py9 coloured by chain, front view.
PDB entry 3py9 coloured by chain, side view.
PDB entry 3py9 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
X-ray structural studies of the entire extracellular region of the serine/threonine kinase PrkC from Staphylococcus aureus.
Ruggiero A, Squeglia F, Marasco D, Marchetti R, Molinaro A, Berisio R
Biochem J 435 33-41 (2011)
PMID: 21208192

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-494107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 32.71 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JZ56 (Residues: 371-664; Coverage: 44%)
Gene name: SAV1220
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EU 4 x EU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21212
Unit cell:
a: 55.681Å b: 69.103Å c: 88.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.244 0.293
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Messenger functions of the bacterial cell wall-derived muropeptides.
Boudreau et al. (2012)
9 more

3 mentions without citation

Bacterial cell division regulation by Ser/Thr kinases: a structural perspective.
Ruggiero et al. (2012)
2 more